| |
|
If you scanned your TEM negatives and want to know the pixel dimensions for quantification, please use the following information as a guide.
Magnification |
1mm = |
Magnification |
1mm = |
2,000X |
500.00nm |
15,000X |
66.67nm |
2,500 |
400.00 |
20,000 |
50.00 |
3,000 |
333.33 |
27,000 |
37.04 |
4,000 |
250.00 |
30,000 |
33.33 |
5,000 |
200.00 |
40,000 |
25.00 |
6,000 |
166.67 |
50,000 |
20.00 |
8,000 |
125.00 |
60,000 |
16.67 |
10,000 |
100.00 |
67,000 |
14.93 |
12,000 |
83.33 |
80,000 |
12.50 |
14,000 |
71.43 |
100,000 |
10.00 |
1 inch = 2.54cm = 25.4mm
To determine the pixel size in nanometers, you must know the scanning resolution and the 1mm equivalent for the magnification used (see table).
If the magnification is not listed in the table above, please see Frank, Leslie or Geoff.
Example:
Negative scanned at 1,000 ppi: mag at 5,000X, 1 mm = 200 nm
Calculation to pixel size:
1,000 ppi
= 39.4 pixels/mm
25.4mm/in
200 nm
= 5.0 nm/pixel
39.4 pixel/mm
In the above example, 200 nm comes from the chart above because the image was taken
at 5,000X.
You need to choose the correct number from the conversion chart above based on the ____X
magnification.
1 um = 1,000 nm
1000 nm
= 200 pixels
5.0 nm/pixel
200 pixels = 1 micron scalebar.
 |