title graphic AIF graphic

STEMI
How big are the thingies in my pictures???

One of the nice things about digital imaging is that it is easy to make figures for publication using Photoshop.  However, it is very important to keep track of the magnification of each image you snap.  You don't want the following problem:

ant-figure768.jpg (102282 bytes)

The magnification of the stereo microscope is highly adjustable by simply turning a knob.  Therefore, you should take a picture of a magnification standard every time you change the magnification of the microscope.

To make this easy, the AIF staff have placed magnification standards next to the microscope and have attached them with strings to reduce the chance of their disappearing.

rulers.jpg (34800 bytes)

Each time you change the magnification, image the appropriate scale with the microscope:

rulers-on-scope.jpg (83573 bytes)

This way, you will have results that you may line up as follows:

scale.jpg (83935 bytes)

And here is an example where a user took a snapshot of the scale at the same time as the sample (rat liver chunks).

rat-pate.jpg (36397 bytes)

Ahhh, now we know how big these chunks of liver really are!
(Really, this picture wasn't taken with the STEMI but with a Sony Cyber-shot DSC-P31 digital snapshot camera, but the point about knowing the scale is the same.)

 

next.jpg (3139 bytes)