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Function Keys:
| F1 | Toggles fluor shutter open/closed. If user changes shutter manually, this button will need to be pressed twice to catch up. |
| F2 | Toggles halogen (brightfield) lamp on/off. If user changes lamp on/off status manually, this button will need to be pressed twice to catch up. Intensity for lamp is set by <Shift>F12. This may range from 0 (black) to 120 (maximum intensity). |
| F3 | Focus Live (There appears to be an error calling the Olympus microscope at the end of this routine; click to ignore the error.) Set binning and exposure time using <Shift>F12. |
| F7 | Focus down 1 um |
| F8 | Focus up 1 um |
| F9 | Quicksnap, use <Shift>F11 to preset |
| F10 | Quicksnap, use <Shift>F11 to preset |
| F11 | Quicksnap, use <Shift>F11 to preset |
| F12 | Quicksnap, use <Shift>F11 to preset |
| <Shift>F11 | Sets the Quciksnap exposure times and binning. |
| <Shift>F12 | Use to: set focus (F3) exposure time and binning and set halogen lamp intensity (used by F2) |
Variables used:
| Montage collection scripts use the following: | |
| V191 | Binning for Focus Live; default set to 2; ; use <Shift>F12 to change. |
| V190 | exposure time for Focus Live; default set to 100; use <Shift>F12 to change. |
| V150 | Field width (um) |
| V151 | Field height (um) |
| V152 | Fields to right (columns to snap) |
| V153 | Fields down (rows to snap) |
| V154 | exposure (ms) |
| V155 | binning |
| V156 | counter for x (columns) |
| V157 | counter for y (rows) |
| V158 | y rewind |
| V140 | Step direction X (-1 or 1) |
| V141 | Step direction X (-1 or 1) |
| V142 | X of starting corner |
| V143 | Y of starting corner |
| V144 | X of ending corner |
| V145 | Y of ending corner |
| V146 | Z of starting corner |
| V147 | Z of ending corner |
| V148 | step direction Z |
| V149 | Z step size = V148 * ( abs(V147-V146) / sqrt(width^2 + height^2) ) |
Scales and Montaging with Prior Stage:
Full 1X1 binning camera size is 1280 X 1024 pixels.
At 20X, the field is 410 um wide. Each pixel = 0.32 um. The height, therefore, is 328 um.
Other Technical Details:
SIIG 4 com port card installed; no COM2 on computer.
Prior stage in COM3; null modem cable; 9600 baud; 8 bits; no parity; 2 stop bits.
Olympus controller in COM1; straight through cable; 9600 baud; 8 bits; no parity; 1 stop bit.
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