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Objective lens power | 10X | 20X | 40X air | 40X oil | 60X | 60X with 1.5X eyepiece | 60X with 1.6X eyepiece |
N.A. | 0.30 | 0.40 | 0.60 | 1.00 | 1.40 | 1.40 | 1.40 |
Pixel size mm | 0.66 | 0.330 | 0.165 | 0.165 | 0.110 | 0.073 | 0.069 |
The values for pixel size are for 1 X 1 binning. If you use 2 X 2 binning, simply multiply by 2. For 4 X 4 binning, multiply by 4.
Objective lens | 1.25X to 40X air | 25X to 100X oil |
Refractive Index | 1.00 | 1.515 |
fluorochrome | DAPI | Low blue | FITC | Rhodamine | Cy5 |
wavelength | 0.460 | 0.460 | 0.520 | 0.600 | 0.685 |
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