title graphic AIF graphic

Numbers for Deconvolution

 

Objective lens power 10X 20X 40X air 40X oil 60X 60X with 1.5X eyepiece 60X with 1.6X eyepiece
N.A. 0.30 0.40 0.60 1.00 1.40 1.40 1.40
Pixel size mm 0.66 0.330 0.165 0.165 0.110 0.073 0.069

The values for pixel size are for 1 X 1 binning.  If you use 2 X 2 binning, simply multiply by 2.  For 4 X 4 binning, multiply by 4.

 

Objective lens 1.25X to 40X air 25X to 100X oil
Refractive Index 1.00 1.515

 

fluorochrome DAPI Low blue FITC Rhodamine Cy5
wavelength 0.460 0.460 0.520 0.600 0.685

 

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