title graphic AIF graphic
Time lapse of cells in culture with Multi-Photon microscope.
MTLn3 cells permanently transfected to express GFP constituatively grown in MatTek coverslip bottom dishes.  Imaged with 60X N.A. 1.4 oil immersion objective on inverted microscope.  Objective heater by Bioptechs and stage heater by World Precision Instruments to keep cells at 37 degrees C.
  • cycle time is 15 seconds; 80 images total for duration of 20 minutes
  • laser 960 nm
  • laser power set in LaserSharp software at 0.5%; low power setting minimizes exposure to light
  • gain at 60%
  • 166lps direct scans at 512 X 512 pixels
  • zoom approx 2X (images resized for web presentation)

Yellow arrows point to rows of chromosomes.

tlapse_jun01_1mincycles.gif (77823 bytes)

 

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Page last revised 11 July 2001 by Michael Cammer