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Example fluorescent images from the Stereo Dissection Microscope with fluorescent adapter

These are the first fluorescent images we took with the new fluorescent attachment to the STEMI.

From John Greally's lab:
D4/XEGFP mice crossed with CSTBL/6 (originally created by Dr. Andras Nagy).
These female mice (5 days old) have an X linked GFP transgene that undergoes maturation in half the cells resulting in a variegated fluorescence.
The pictures were taken with a 1.6X objective.  Also the microscope has a 1X and a 0.6X objective so that it should be able to image a larger field than shown here.

Here is the top of a mouse's head:
fluor_head.jpg (93370 bytes)

 

The mouse on the bottom is GFP expressing.  The mouse in the top right corner is barely visible because it is GFP negative.
pos_neg_mouse.jpg (107315 bytes)

 

The paw on the left is of a GFP negative mouse.  The paw on the right is of a GFP expressing mouse.
paws.jpg (50863 bytes)

After the mice were imaged they were returned to their mother for further maturation and breeding.

return to index next.jpg (3139 bytes)