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3D confocal microscopy
| Immunolocalization of Golgi in GH3 cells. Cells were grown on polylysine-coated
coverslips, fixed with 3% paraformaldehyde, and permeabilized with 0.2% saponin. They were
immunostained for antigens specific to Golgi. Images were collected with a BioRad MRC 600 laser scanning confocal microscope at the Analytical Imaging Facility. Maximum pixel projections for the 360 degree rotation were performed with NIH-Image. The quicktime movie (which you should see spinning at the left) was made with Adobe Premier. Images courtesy of Drs. Dennis Shields and Yeguang Chen. For more, see Chen, Y.G., Danoff, A. and Shields, D. (1995) The propeptide of anglerfish preprosomatostatin-I rescues prosomatostatin-II from intracellular degradation. J Biol. Chem. 270:31, 18598-18605. Visit Dr. Dennis Shields at http://shieldslab.aecom.yu.edu/ |
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