|Leica AOBS SP2 confocal microscopy
The Leica confocal is one of a few
laser scanning confocal microscopes in the AIF. The Leica may be programmed to
scan many different wavelength ranges. The Leica AOBS uses variable spectral
detection instead of traditional emission filters. There are a few laser lines for
excitation and the dyes are limited by these. Any dye that is excited at one of
these following wavelengths can be used: 405; 458; 476; 488;
514; 561; and 633 nm. According to the sales literature, the
lasers are: diode 20 mW 405 nm; Ar 100 mW 457 nm, 488 nm, 514 nm; diode 10mW 561 nm;
HeNe 10 mW 633 nm.
63X N.A. 1.4-0.60 Oil lBL HCX PL APO
40X N.A. 1.25-0.75 Oil CS HCX PL APO
20X N.A. 0.70 1mm corr lBL HC PL APO
Automated stage for tiling XY, XYZ or XYT volumes with 166.6
mm of travel in Z.
Here's a picture of the new system as installed at the AIF on
November 6, 2002.
Leica Application Notes:
Sample method section for a paper:
Images were collected with a Leica TCS SP2 AOBS confocal microscope
(Mannheim, Germany) with 25X and 60X oil immersion optics. Laser lines at 488nm and 543nm
for excitation of Cy2 and Cy3 were provided by an Ar laser and a HeNe laser. Detection
ranges were set to eliminate crosstalk between fluorophores.
- Azios, NG, Krishnamoorthy, L, Harris, M., Cubano, LA, Cammer, M,
Dharmawardhane, SF. Estrogen and Resveratrol Regulate Rac and Cdc42 Signaling
to the Actin Cytoskeleton of Metastatic Breast Cancer Cells. Neoplasia. 2007 Feb;
- Bhatia S, Edidin M, Almo SC, Nathenson SG. (2005) Different cell
surface oligomeric states of B7-1 and B7-2: Implications for signaling. Proc Natl Acad
Sci U S A. 102(43):15569-15574. PMID: 16221763
- Eugenin EA, Berman JW. Gap junctions mediate human
immunodeficiency virus-bystander killing in astrocytes. J Neurosci. 2007 Nov
21;27(47):12844-50. PMID: 18032656
- Herskovits AZ, Davies P. (2006) The regulation of tau phosphorylation
by PCTAIRE 3: Implications for the pathogenesis of Alzheimer's disease. Neurobiol Dis.
2006 Jun 9; [Epub ahead of print] PMID: 16766195
- Lazar-Molnar E, Almo SC, Nathenson SG. The interchain disulfide
linkage is not a prerequisite but enhances CD28 costimulatory function. Cell Immunol. 2007
Apr 27; [Epub ahead of print] PMID: 17467674
- Maxson, ME, Cook, E, Casadevall, A, Zaragoza, O. The volume and
hydration of the Cryptococcus neoformans polysaccharide capsule. Fungal Genetics and
Biology. March 2007; 44(3)180-186
- Shav-Tal Y, Darzacq X, Shenoy SM, Fusco D, Janicki SM, Spector DL,
Singer RH. (2004) Dynamics of Single mRNPs in Nuclei of Living Cells. Science
- Zhang X, Schwartz JC, Guo X, Bhatia S, Cao E, Lorenz M,
Cammer M, Chen L, Zhang ZY, Edidin MA, Nathenson SG, Almo SC. (2004) Structural and
functional analysis of the costimulatory receptor programmed death-1. Immunity.